ZytoLight® products are designed for the identification of genetic aberrations, e.g. translocations, deletions, amplifications, and chromosomal aneuploidies by Fluorescence in situ Hybridization (FISH) in formalinfixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.
ZytoLight
Method Description
​The ZytoLight® System uses direct labeled FISH probes, eliminating the need to detect the probes with fluorophore-coupled- antibodies. The probes are detected by fluorescence microscopy using appropriate filter sets. Due to an exciter filter, full-spectrum light, emitted by the microscope lamp, is reduced to light of a defined wavelength that specifically excites the fluorophore of the probe. This light is reflected onto the specimen by a dichroic mirror. The fluorophore emits light of longer wavelengths that passes the mirror. Finally, a barrier filter reduces the emitted light to a defined wavelength that can be detected.
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High Sensitivity and Specificity
ZytoLight® FISH probes are direct labeled using the unique ZytoLight® Direct Label System II providing improved signal intensity. All ZytoLight® single copy (SPEC) probes are processed by the unique ZytoVision® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!

ZytoLight® SPEC and CEN Probes
ZytoLight® SPEC probes hybridize to specific, single copy DNA sequences of the human genome. ZytoLight® SPEC probes are available for the detection of a variety of chromosomal aberrations associated with tumors and genetic diseases.
ZytoLight® CEN Probes hybridize to highly repetitive human satellite DNA sequences located at the centromeric regions of chromosomes producing sharp, bright signals specific for each individual chromosome.


For further literature on any of the probes mentioned below please contact us here
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​Chromosome 1
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Prod. No. Product Locus Label

Z-2019 ZytoLight RMS II Probe
SPEC t(1;13) Dual Color Fusion Probe 1p36/13q14 green/orange
Z-2075 ZytoLight SPEC 1p36/1q25 Dual Color Probe 1p36.32/1q25.3 orange/green
Z-2080 ZytoLight SPEC MDM4/1p12 Dual Color Probe 1q32.1/1p12 green/orange

Chromosome 2​
Prod. No. Product Locus Label
Z-2018 RMS I Probe
SPEC t(2;13) Dual Color Fusion Probe 2q36/13q14 green/orange
Z-2057 SPEC HER4/2q11 Dual Color Probe 2q34/2q11.2 green/orange
Z-2049 SPEC 2q11 Probe 2q11.2 orange
Z-2074 SPEC NMYC/2q11 Dual Color Probe 2p24/2q11.2 green/orange
Z-2117 SPEC ALK/EML4 TriCheck Probe 2p23/2p23/2p21 green/orange/blue
Z-2124 SPEC ALK Dual Color Break Apart Probe 2p23 green/orange
Z-2127 SPEC SOX2/CEN 3 Dual Color Probe 3q26 green/orange
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Chromosome 3
Prod. No. Product Locus Label
Z-2001 ZytoLight CEN 3 Probe (Alpha Sat. D3Z1) 3p11.1-q11.1 orange
Z-2062 ZytoLight SPEC FHIT/CEN 3 Dual Color Probe 3p14.2/3p11.1-q11.1 green/orange
Z-2084 ZytoLight SPEC VHL/CEN3 Dual Color Probe 3p25.3/3p11.1-q11.1 green/orange
Z-2124 SPEC ALK Dual Color Break Apart Probe 2p23 green/orange
Z-2127 SPEC SOX2/CEN 3 Dual Color Probe 3q26 green/orange
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Chromosome 4
Prod. No. Product Locus Label
Z-2082 ZytoLight SPEC FGFR3/CEN 4 Dual Color Probe 4p16.3/4p11.1-q11.1 green/orange
Z-2083 ZytoLight CEN 4 Probe 4p11.1-q11.1 orange
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Chromosome 5
Prod. No. Product Locus Label
Z-2091 ZytoLight SPEC TERT/5q31 Dual Color Probe 5p15.33/5q31 green/orange
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Chromosome 6
Prod. No. Product Locus Label
Z-2002 ZytoLight CEN 6 Probe (Alpha Sat. D6Z1) 6p11.1-q11.1 green
Z-2069 ZytoLight SPEC ESR1/CEN 6 Dual Color Probe 6q25/6p11.1-q11.1 green/orange
Z-2070 ZytoLight SPEC ESR1/CEN 6 Dual Color Probe Kit 6q25/6p11.1-q11.1 green/orange
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Chromosome 7
Prod. No. Product Locus Label
Z-2003 ZytoLight CEN 7 Probe (Alpha Sat. D7Z1) 7p11.1-q11.1 orange
Z-2033 ZytoLight SPEC EGFR/CEN 7 Dual Color Probe 7p12/7p11.1-q11.1 green/orange
Z-2053 ZytoLight SPEC EGFR/CEN 7 Dual Color Probe Kit 7p12/7p11.1-q11.1 green/orange
Z-2054 ZytoLight SPEC EGFR Probe 7p12 green
Z-2087 ZytoLight SPEC MET/CEN 7 Dual Color Probe 7q31.2/7p11.1-q11.1 green/orange
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Chromosome 8
Prod. No. Product Locus Label​
Z-2004 ZytoLight CEN 8 Probe (Alpha Sat. D8Z2) 8p11.1-q11.1 green
Z-2072 ZytoLight SPEC FGFR1/CEN 8 Dual Color Probe 8p12/8p11.1-q11.1 green/orange
Z-2090 ZytoLight SPEC CMYC Dual Color Break Apart Probe 8q24 green/orange
Z-2092 ZytoLight SPEC CMYC/CEN 8 Dual Color Probe 8q24/8p11.1-q11.1 green/orange

Chromosome 9
Prod. No. Product Locus Label​
Z-2063 ZytoLight SPEC p16/CEN 9 Dual Color Probe 9p21/9q12 green/orange
Z-2067 ZytoLight CEN 9 Probe (Classical Sat. III D9Z3) 9q12 orange
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Chromosome 10
Prod. No. Product Locus Label​
Z-2079 ZytoLight CEN 10 Probe (Alpha Sat. D10Z1) 10p11.1-q11.1 orange
Z-2078 ZytoLight SPEC PTEN/CEN 10 Dual Color Probe 10q23/10p11.1-q11.1 green/orange
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Chromosome 11
Prod. No. Product Locus Label​
Z-2005 ZytoLight CEN 11 Probe (Alpha Sat. D11Z1) 11p11.1-q11.1 green
Z-2014 ZytoLight MEC I Probe SPEC t(11;19)
Dual Color Break Apart Probe 11q21 green & orange
Z-2071 ZytoLight SPEC CCND1/CEN 11 Dual Color Probe 11q13/11p11.1-q11.1 green/orange
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Chromosome 12
Prod. No. Product Locus Label​
Z-2013 ZytoLight SPEC MDM2/CEN 12 Dual Color Probe 12q14.3-15/12p11.1-q11.1 green/orange
Z-2025 ZytoLight SPEC MDM2/CEN 12 Dual Color Probe Kit 12q14.3-15/12p11.1-q11.1 green/orange
Z-2050 ZytoLight CEN 12 Probe (Alpha Sat. D12Z3) 12p11.1-q11.1 green
Z-2056 ZytoLight SPEC HER3/CEN 12 Dual Color Probe 12q13/12p11.1-q11.1 orange/green
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Chromosome 13​
Prod. No. Product Locus Label​
Z-2018 ZytoLight RMS I Probe SPEC t(2;13) Dual Color Fusion Probe 2q36/13q14 green/orange
Z-2019 ZytoLight RMS II Probe SPEC t(1;13) Dual Color Fusion Probe 1p36/13q14 green/orange
Z-2085 ZytoLight SPEC t(1;13) Dual Color Fusion Probe 1p36/13q14 orange
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Chromosome 17
Prod. No. Product Locus Label​
Z-2006 ZytoLight CEN 17 Probe (Alpha Sat. D17Z1) 17p11.1-q11.1 orange
Z-2011 ZytoLight SPEC HER2 Probe 17q12 green
Z-2015 ZytoLight SPEC HER2/CEN 17 Dual Color Probe 17q12/17p11.1-q11.1 green/orange
Z-2020 ZytoLight SPEC HER2/CEN 17 Dual Color Probe Kit 17q12/17p11.1-q11.1 green/orange
Z-2073 ZytoLight SPEC HER2/TOP2A/CEN 17 Triple Color Probe 17q12/17q21/17p11.1-q11.1 green/red/gold
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Chromosome 18
Prod. No. Product Locus Label​
Z-2007 ZytoLight CEN 18 Probe (Alpha Sat. D18Z1) 18p11.1-q11.1 orange

Chromosome 19​
Prod. No. Product Locus Label​
Z-2076 ZytoLight SPEC 19q13/19p13 Dual Color Probe 19q13.32/19p13.3 orange/green
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Chromosome 21​
Prod. No. Product Locus Label​
Z-2086 ZytoLight SPEC 21q22 Probe 21q22 orange
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Chromosome X​
Prod. No. Product Locus Label ​
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Z-2008 ZytoLight CEN X Probe (Alpha Sat. DXZ1) Xp11.1-q11.1 orange
Z-2016 ZytoLight CEN X/Y Dual Color Probe Xp11.1-q11.1/Yq12 orange/green
Z-2089 ZytoLight CEN Y/X Dual Color Probe Yq12/Xp11.1-q11.1 orange/green
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Chromosome Y​
Prod. No. Product Locus Label

Z-2010 ZytoLight CEN Y Probe (Classical Sat. III DYZ1) Yq12 green
Z-2016 ZytoLight CEN X/Y Dual Color Probe Xp11.1-q11.1/Yq12 orange/green
Z-2089 ZytoLight CEN Y/X Dual Color Probe Yq12/Xp11.1-q11.1 orange/green