Method Description



The ZytoDot ® System uses Digoxigenin-labelled probes which are, after blocking, detected using a Mouse-anti-Digoxigenin antibody. This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody. The enzymatic reaction of DAB leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.



​

















​







​Method Description – ZytoDot ® 2C



The ZytoDot ® 2C System uses Digoxigenin- and DNP-labeled probe cocktails targeting different genomic sections (1). After blocking (2), the probes are detected by using a Mouse-anti-Digoxigenin/Rabbit-anti-DNP antibody-cocktail (3). These antibodies are detected by polymerized HRP-Goat-anti-Mouse (4) and Ap-Goat-anti-Rabbit (5) antibodies. The enzymatic reaction of AP-red (6) and HRP-green (7) leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.



























​



CISH: A viable alternative to FISH



High concordance between CISH and FISH ranging from 92-100% has
been shown by numerous international studies for HER2 amplification.



Advantages of CISH over FISH



• Quick and easy interpretation of results comparable to IHC
• Simultaneous observation of tissue morphology and CISH signals
• Storage of slides at room temperature – CISH signals are permanent
• No costly fluorescent microscope needed

​

Advantages of ZytoDot ® 2C



• Two targets detected simultaneously
• High contrasting distinct red and green signals
• Visualization at 40x using light microscopy
• Standardized and complete kit solutions

​

High Signal-to-Noise Ratio



All ZytoDot ® probes are processed by the unique ZytoVision ® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!

​