The ZytoDot ® System uses Digoxigenin-labelled probes which are, after blocking, detected using a Mouse-anti-Digoxigenin antibody. This antibody is detected by a polymerized HRP-Goat-anti-Mouse antibody. The enzymatic reaction of DAB leads to the formation of strong permanent brown signals that can be visualized by light microscopy using a 40x objective.
Method Description – ZytoDot ® 2C
The ZytoDot ® 2C System uses Digoxigenin- and DNP-labeled probe cocktails targeting different genomic sections (1). After blocking (2), the probes are detected by using a Mouse-anti-Digoxigenin/Rabbit-anti-DNP antibody-cocktail (3). These antibodies are detected by polymerized HRP-Goat-anti-Mouse (4) and Ap-Goat-anti-Rabbit (5) antibodies. The enzymatic reaction of AP-red (6) and HRP-green (7) leads to the formation of strong permanent red respectively green signals that can be visualized by light microscopy using a 40x objective.
CISH: A viable alternative to FISH
High concordance between CISH and FISH ranging from 92-100% has
been shown by numerous international studies for HER2 amplification.
Advantages of CISH over FISH
• Quick and easy interpretation of results comparable to IHC
• Simultaneous observation of tissue morphology and CISH signals
• Storage of slides at room temperature – CISH signals are permanent
• No costly fluorescent microscope needed
Advantages of ZytoDot ® 2C
• Two targets detected simultaneously
• High contrasting distinct red and green signals
• Visualization at 40x using light microscopy
• Standardized and complete kit solutions
High Signal-to-Noise Ratio
All ZytoDot ® probes are processed by the unique ZytoVision ® Repeat Subtraction Technique resulting in advanced specificity and less background. No further blocking of repetitive sequences is needed!
ZytoDot ® products are designed for the detection of aneuploidies and gene amplifications by Chromogenic in situ Hybridization (CISH) in formalinfixed, paraffin-embedded tissue sections, cell samples, blood or bone marrow smears, and metaphase chromosome spreads.